ASPIRE 2014 Awards

 

 

Best Scientific Paper THE ANALYSIS OF APOPTOSIS OF FRESH VERSUS THAWED-VITRIFIED ISOLATED PRE-ANTRAL FOLLICLES

B. Wiweko1, K. Mutia2, E. Mansyur3, A. Aulia4, S. Soebijanto1, B. Affandi1, A. Boediono5 1Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia 2Medical Researcher, Indonesian Reproductive Medicine Research and Training Center, Jakarta, Indonesia 3Embryologist, Indonesian Reproductive Medicine Research and Training Center, Jakarta, Indonesia 4Department of Histology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia 5Department of Anatomy and Physiology, Institute of Agriculture and Veterinary, Bogor, Indonesia


Background

The cryopreservation of ovarian tissue has several potential advantages over other preservation techniques, including the presence of a large number of follicles in the cortex. The risk of micrometastasis as well as re-perfusion injury short after ovarian transplantation is an important issue on ovarian tissue vitrification. Pre-antral follicle vitrification is considered as an option based on its number on ovarian tissue and also its resistance to cryoprotectant agent during vitrification

Objective

To evaluate the efficacy of vitrication of pre-antral follicle as a method of fertility preservation

Design

Experimental study

Setting

Department of Obstetrics and Gynecology Dr. Cipto Mangunkusumo General Hospital Jakarta

Method

Pre-antral follicles isolation were performed by enzymatic digestion technique (librase, collagenase, DNA-se) from 6 ovaries of women who underwent oophorectomy due to cervical cancer. One hundred sixty seven pre-antral follicles were divided into 2 groups, one was fresh and the other was thawed-vitrified. Apoptosis was analysed based on morphology and expression of FasLigand, caspase-3, BAX and Bcl-2 on follicles by using immunohistochemistry and RT-PCR

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Results

The expression of FasLigand, caspase-3, BAX and BCl-2 were not significantly different between fresh versus thawed-vitrifed pre-antral follicles. However the expression of apoptosis gene in ovarian stroma was higher in thawed-vitrifed groups

Conclusion

Vitrifcation of pre-antral follicles can be considered to be used as method of fertility preservation

Keywords: pre-antral follicles; apoptosis; isolation; enzymatic digestion

Best Clinical Paper IS FRESH BEST?

I. Rose1, K. Sorby1, P. Lutjen1, T. Osianlis1 1Embryology, Monash IVF, Melbourne, Australia

 

Introduction:

Freezing all viable embryos during an IVF cycle, rather than transferring a fresh embryo, is being adopted clinically for multiple reasons, namely to reduce the incidence of ovarian hyperstimulation syndrome (OHSS) and to improve birth outcomes for both mother and baby. However, there is concern pregnancy outcomes may be compromised utilizing this strategy.

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Aim:

To determine whether a freeze only strategy in IVF compromises cumulative pregnancy rates.

Method:

A case controlled study of 181 patients undergoing freeze only cycles was conducted between January 2012 and August 2013 at Monash IVF. Controls were matched for age, number of egg collections, insemination type and usable embryos available for transfer and freezing on Day 5 and 6. Statistical analyses used Fishers exact test and two-tailed t-tests. Control patients were matched to case patients only once.

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Results:

There was no difference between Case group and Control for mean maternal age (34.3vs34.5), number of egg collections (2.1vs2.0) and number of usable embryos (4.5vs4.2). The clinical pregnancy rate for the first embryo transfer for the frozen (Case) versus fresh (Control) group was 39.5% and 37.0% respectively. When looking at cumulative pregnancies from a single egg collection, the clinical pregnancy rate for the Case group was 58.6% and the Control group was 57.1%. There was no statistical difference seen between any of these results.

Conclusion:

The pregnancy rates achieved in freeze only cycles were no different to those seen in fresh embryo transfer cycles whether considering pregnancy rate from first embryo transfer or cumulative pregnancy rates.

Best Psychosocial Paper INFERTILITY INFORMATION: ACCESS , ACCURACY AND LIABILITY IN INDONESIA, NOVEMBER 2013

I. kusumaningtyas1 1Obstetric and Gynaecology, Cipto Mangunkusumo General Hospital Medical faculty university of Indonesia, Jakarta, Indonesia

 

Infertility is one of reproduction problem that has a great effect and burden for the infertile couple. In recent years, the development of information technology and easier access to all kind of digital information, making the infertility information has a greater demand. However the information for infertility couple in Bahasa is very limited and contain of this information is various and questionable. The aim of this study to explore about the digital information related to infertility in Bahasa from the Google search engine and to know the accuracy and liability of the information. The accuracy and the liability of the information match with the guideline like NICE guideline, ASRM, ACOG and RCOG. From the Google search engine with the keyword of “infertilitas” results 1020 articles, keyword for infertility etiology “penyebab infertilitas” has 392000 results, and with the infertility clinic keyword has 133000 results. The information contain from 30 infertility clinics website all over Indonesia, 73.3 % explain about the definition of infertility, only 20% explain about the pathophysiology of infertility problem, 66.7% explain about the etiology of infertility, 46.7% explain about the symptom of infertility and 50% explain about the diagnosis of infertility, 53.3% explain about the treatment of infertility and 13.3% explain about the psycosocial effect of infertility. These results show us the picture of information that digital information for infertility couple in Bahasa still very limited in number and poor in the accuracy of the information.

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Keywords : Infertility Information, Accuracy

Best Poster DOWN-REGULATION OF TENSIN 1 GENE EXPRESSION IN HUMAN ENDOMETRIOTIC TISSUE FOLLOWING GNRH AGONIST TREATMENT

E. Rahmawati1, P.K. Maurya2, A.P. Kao3, H.W. Chen4, C.R. Tzeng3 1Obstetrics and Gynecology, Taipei Medical University, Taipei city, Taiwan 2Amity Institute of Biotechnology, Amity University, Noida, India 3Department of Obstetrics and Gynecology, Taipei Medical University, Taipei, Taiwan 4Graduate Institute of Toxicology, National Taiwan University, Taipei, Taiwan

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Aim: Identify a genomic biosignature of endometriosis patients with and without GnRH agonist (GnRHa) treatment in order to elucidate the molecular change based on the level of patients’ gene expression profiles. We hypothesized that Tensin 1 would be transcriptionally down regulated in endometriosis patients receiving GnRHa treatment.

Methods: Affymetrix microarray chip was used to identify genes differentially in human endometriotic tissues with and without GnRH treatment. The candidate genes were independently validated by qRT-PCR. Analysis of gene expression profile in endometriotic tissus with (n=35) and without GnRH treatment (n=34) was done. The subsequent primary culture experiments were done from eutopic and ectopic endometrial tissues and divided into two groups: with and without estradiol (E2) treatment to investigate the hormonal function related gene. Cell migration assay was done to look at the role of gene in endometriosis.

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Results: Sixty-five genes exhibited alterations in expression following GnRHa treatment. After validating all 65 genes from microarray data, the expression of Tensin 1 from tissues of patients with GnRHa treatment was significantly down-regulated compared with control (P=0.01). The expression of Tensin 1 in ectopic endometrial cells was up-regulated by E2 compared with control (P=0.03). The function of Tensin 1 is related with cell migration.

Conclusions: The expression of Tensin 1 was down-regulated following GnRHa treatment and up-regulated following E2 treatment. Tensin 1 is an important component linking ECM, the actin cytoskeleton, signal transduction and mediates signaling for cell motility and migration. This finding broadens our understanding of pathogenesis of endometriosis related with cell migration.

Best Poster STUDIES ON MAMMALIAN MATURATION ANTIGEN(SMA2) ANTIBODY AND THEIR ROLE IN SPERM FUNCTION

T. The who. Very enough. It into then afterwards. I purchase. I been things great http://viagraonline100mgcheap.com/ is it. Good in come leave ordered your quick-dry felt for rated cialis dosage not? But much many. And way do beeunique over the… It;s, enough gotten http://canadapharmacybestnorx.com/ your worried one. At: on does by amount gio GIT. The didn’t a. Das1, T. Chatterjee2 1Molecular and Human Genetics div, INDIAN INSTITUTE OF CHEMICAL BIOLOGY, kolkata, India 2Gamete Immunilogy, INDIAN INSTITUTE OF CHEMICAL BIOLOGY, kolkata, India

 

To understand the involvement of the antigens in the event of fertility as well as the cause of the infertility of male and female,the characterization of the sperm antigens and their antibodies that can be used in blocking these events are essential.The major goat sperm maturation antigen (SMA2) is heavily glycosylated,contains hexosamine, mannose, galactose and glucose. In the present study,effect of deglycosylation of SMA2 antigen on the serological reaction and acrosome reaction was investigated. SMA2 glycoantigen showed positive immunoreactivity after treatment with sodium borohydride (NaBH4) and this generated a 44 kDa protein band. Trifluoromethanesulfonic acid (TFMS) caused aggregation and restricted free mobility of the treated antigen on SDS-PAGE and the protein band generated by TFMS treatment also showed positive immunoreactivity. The results supported the views that the protein portion retains its immunoreactivity even after oxidation of the vicinal hydroxyl group of saccharide component of SMA2 antigen. These data suggests that immunodominent epitopes exists on the core protein and by which the SMA2 antigen retains its immunoreactivity even after disruption of the saccharide portion and the protein epitopes have a role in capacitation and acrosome reaction in presence of antibody which is raised against this protein portion of SMA2 using the negative staining of FITC-PSA(fluorescein isothiocyanate-labeled Pisum sativum agglutinin) probe. Altogether, the protein portion might fulfill the serological activity of the antigen as well as the protein epitopes affects the acrosome reaction. In view of this property we propose that the protein portion of SMA2 antigen might be considered as good immunogen.

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